This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Fabre, O Articles by Fabiani, J N Search for Related Content PubMed PubMed Citation Articles by Fabre, O Articles by Fabiani, J N Pubmed/NCBI databases Substance via MeSH Hazardous Substances DB HEPARIN Social Bookmarking                         What's this?

A recovery model of partial cardiopulmonary bypass in the rat

Department of Thoracic and Cardiovascular Surgery, Broussais Hospital, Paris, o-fabre{at}chru-lille.fr

R Zegdi

Department of Thoracic and Cardiovascular Surgery, Broussais Hospital, Paris

A Vincentelli

Department of Cardiac Surgery, Cardiologic Hospital, Lille

M Cambillaud

Department of Biochemistry, Broussais Hospital, Paris

A Prat

Department of Cardiac Surgery, Cardiologic Hospital, Lille

A Carpentier

Department of Thoracic and Cardiovascular Surgery, Broussais Hospital, Paris

J N Fabiani

Department of Thoracic and Cardiovascular Surgery, Broussais Hospital, Paris

This study was undertaken to develop a recovery model of cardiopulmonary bypass (CPB) in rats. Twenty male Wistar rats (475-550 g) were anaesthetized, mechanically ventilated and the femoral vessels cannulated. The extracorporeal circulation circuit comprised a roller pump, a venous reservoir and a modified Capiox 308 paediatric membrane oxygenator. Priming consisted of 20 ml of fresh homologous blood and 15 ml of colloid. Anticoagulation was achieved with heparin (500 IU/kg). Blood gas analysis, blood pressure monitoring and survival studies were performed in CPB (n=10) and Sham (n=10) rats.

Partial CPB was always easily established and was conducted at a flow rate of 100 ml/kg/min for 90 min. Blood gas analysis and blood pressure data did not differ between the two groups. All CPB rats survived and the 3-week follow-up period remained uneventful.

The rat model of CPB was easy to perform and was associated with excellent survival. This recovery model should allow us to study the pathophysiological processes underlying post-CPB multiple organ dysfunction.

Perfusion, Vol. 16, No. 3, 215-220 (2001)
DOI: 10.1177/026765910101600307


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				F. Doguet, P.-Y. Litzler, F. Tamion, V. Richard, M.-F. Hellot, C. Thuillez, A. Tabley, F. Bouchart, and J. P. Bessou Changes in mesenteric vascular reactivity and inflammatory response after cardiopulmonary bypass in a rat model Ann. Thorac. Surg., June 1, 2004; 77(6): 2130 - 2137. [Abstract] [Full Text] [PDF]

				U. Moehrlen, U. Stammberger, C. Moehrlen, and R. A. Schmid Partial cardiopulmonary bypass in rats using a hollow fibre oxygenator Interactive CardioVascular and Thoracic Surgery, December 1, 2003; 2(4): 603 - 606. [Abstract] [Full Text] [PDF]

				R. Zegdi, O. Fabre, N. Lila, P. Fornes, M. Cambillau, M. Shen, P. Herve, A. Carpentier, and J.-N. Fabiani Exhaled carbon monoxide and inducible heme oxygenase expression in a rat model of postperfusion acute lung injury J. Thorac. Cardiovasc. Surg., December 1, 2003; 126(6): 1867 - 1874. [Abstract] [Full Text] [PDF]